Identification of bZIP gene family of Paulownia fortunei and their response to pathogenic process of witches’ broom phytoplasmasChinese Full TextEnglish Full Text (MT)
DONG Yang;DENG Minjie;MO Shuangfeng;FAN Guoqiang;College of Forestry, Henan Agricultural University;
Abstract: 【Objective】The purpose of our study was to identify the basic leucine zipper（bZIP） gene family in the genome of Paulownia fortunei, and to study the role of this gene family member in the occurrence of Paulownia witches’ broom（PaWB）. 【Method】Based on the genome map of P. fortunei, the PfbZIP gene family members were identified with E-value＜0.001 as the threshold by using hidden Markov model, Arabidopsis bZIP protein sequences and BlastP program; the MAGE7.0 software was used to perform protein sequence alignment and phylogenetic tree construction, and Tbtools software was used to analyze gene structure and promoter cis-acting elements. The PfbZIP genes related to PaWB occurrence were screened out through the comparison of transcriptome data before and after pathogenetic process as well as the recovery process of PaWB. 【Result】A total of 89 PfbZIP gene family members were identified, which were divided into 13 subfamilies according to phylogenetic analysis; the collinearity analysis revealed that 63 fragment duplication events occurred in this family; cis-element analysis showed that the PfbZIP genes family might have a response to biological processes such as light, plant hormone regulation, biotic and abiotic stresses. Comparative analysis of the RNA-seq data before and after pathogenetic process as well as the recovery process of PaWB revealed that 30 PfbZIP genes, including PfbZIP46, were significantly differentially expressed before and after the occurrence of PaWB. Furthermore, the expression level of PfbZIP46 gene also continuously changed during the recovery process of PaWB-infected seedlings. Therefore, it was speculated that PfbZIP46 might be related to the process of phytoplasma infection. 【Conclusion】A total of 89 PfbZIP genes in the genome of P. fortune was identified. The fragment duplication event may be a significant reason for the expansion of the PfbZIP transcription factor family, and PfbZIP46 may play an important role in the process of phytoplasma infection.
Keywords：
Paulownia fortunei; paulownia witches’ broom（PaWB）; basic leucine zipper（bZIP）; gene expression; evolutionary analysis;
- DOI：
10.16445/j.cnki.1000-2340.20221102.001
- Series：
(D) Agriculture
- Subject：
Plant Protection; Forestry
- Classification Code：
S763.7
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