Preparation and application of multi-effects microbial fertilizer by solid fermentation with corn stalksChinese Full TextEnglish Full Text (MT)
ZHENG Shihao;CHENG Qi;BAO Yongming;Dalian University of Technology,School of Ocean Science and Technology;
Abstract: The nitrogen-fixing bacteria N3（Arthrobacter sp. ZSH N3）, organic phosphorus solubilization bacteria Y1（Enterobacter asburiae ZSH Y1）, inorganic phosphorus solubilization bacteria W3（Enterobacter hormaechei ZSH W3） and bio-control bacteria KY2（Bacillus methylotrophicus KY2） were screened by measuring the ability of nitrogen fixation, phosphorus solubilization and resistance to Fusarium proliferatum. The parameters of solid state fermentation technology of corn stalks were optimized by single factor and response surface experiments with the total number of live bacteria as index. The optimal conditions predicted by response surface experimental results were fermentation temperature of 30.4 ℃, initial pH of 7.8, initial water content of 100%, inoculum of 15.5%, inoculation interval time of 24 h and fermentation time of 120 h. Under the optimal conditions, the total actual effective viable bacteria number of N3, Y1, W3 and KY2 in the microbial fertilizer were up to 2.27×10~9 CFU·g-1, 3.11×10~9 CFU·g-1, 7.10×10~8 CFU·g-1 and 3.21×10~9 CFU·g-1, respectively. The content of total nitrogen and available phosphorus were 6.07 and 11.43 folds compare with the raw materials. The pot experiments showed that the microbial fertilizer could significantly promote the tobacco photosynthesis and effectively inhibited the wilt caused by Fusarium proliferatum.
Keywords：
multi-effects microbial fertilizer; nitrogen-fixing bacteria; phosphorus solubilization bacteria; biological control; solid state fermentation; corn stalks;
- DOI：
10.16445/j.cnki.1000-2340.2020.03.013
- Series：
(D) Agriculture
- Subject：
Fundamental Science of Agriculture; Agronomy
- Classification Code：
S144
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