Journal of Henan Agricultural University. 2018, (02) Core

Effect of laccase on catalytic efficiency of cellulaseChinese Full Text

WANG Xu;HOU Huawei;SI Lingyu;YANG Sen;CHEN Hongge;College of Life Science,Henan Agricultural University;

Abstract: In order to investigate the effect of laccase on catalytic efficiency of cellulase,a comparative analysis of sugar yield from cellulosic hydrolysate of filter paper and corn stover using different addition level of the pure laccase（ Sigma） was performed in this study. The results indicated that the addition of lactase had no significant effect on the sugar yield in the filter paper hydrolysis system which contained cellulase. However,the addition of lactase could decrease the sugar yield（ from 30. 40% to 23. 87%）obviously in the corn stover hydrolysis system（ preincubating laccase and corn stover together at 37 ℃for 24 h）. A negative correlation between the sugar yield and the addition of lactase in the corn stover hydrolysis system was also observed. Hence,laccase had played an inhibitory role in cellulase catalysis in the corn stover hydrolysis system.

Keywords：

laccase; cellulase; sugar yield; corn stover;

DOI：
10.16445/j.cnki.1000-2340.2018.02.015
Series：
(D) Agriculture; (A) Mathematics/ Physics/ Mechanics/ Astronomy; (C) Architecture/ Energy/ Traffic/ Electromechanics, etc
Subject：
Biology; New Energy; Agricultural Engineering
Classification Code：
Q814.9;S216

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Effect of laccase on catalytic efficiency of cellulaseChinese Full Text

WANG Xu;HOU Huawei;SI Lingyu;YANG Sen;CHEN Hongge;College of Life Science,Henan Agricultural University;

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